Bela P. Bozsik, M.D.
Budapest, Hungary

The results of the test are not influenced by either the genetic polymorphism or the phenotype of Borrelia burgdorferi sensu lato (or the changes in either of these during the pathogenesis of the disease), which is very important in Middle-Europe. The test is also reliable in the cases when Lyme disease is caused by new subspecies or immunological changes or when vaccination has produced an antibody response. The theoretical sensitivity of this procedure can be as high as one organism/ml.

Concurrent PCR tests appear to be less sensitive even nowadays. If properly evaluated, it is the most sensitive of the direct tests and it is also very reliable. It provides a tool to investigate natural phenomena in a laboratory setting, which may help to gather new information regarding the pathogenesis of Lyme disease.

In the microscopy, immobile cellular bodies are always seen in the background serving as controls of the moving Spirochetes. DualDur® -treated normal or washed blood or Borrelia burgdorferi sensu lato cultures can also be used as controls. Indirect immunofluorescent assays using specific monoclonal antibodies kindly donated by prof.Barbour and Barbara Johnson prove that the Spirochetes are identical to Borrelia burgdorferi sensu lato. They were proven by electron microscopy in the last years with both negative staining and immuncytologic reactions.  

This reagent provides conditions similar to the body, therefore the division of Spirochetes was detected several times; sometimes they were acting in pictures. You can see it in the following:

The next story will be on the emerging combined antibiotic treatments developed in 1990 and used widely with success in Hungary prescribed by the members of the Therapeutic Workgroup of the Lyme Borreliosis Foundation in Hungary. According to our practice we can tell you that the following could not be proved in Middle Europe: “Conclusion : Patients with post-treatment chronic Lyme disease who have symptoms but show no evidence of persisting Borrelia infection do not show objective evidence of cognitive impairment. Additional antibiotic therapy was not more beneficial than administering placebo." END

Dr. W. Burgdorfer, Dr. B.P. Bozsik and colleague
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”BOZSIK, BELA PAL, retired physician; born in Budapest, Hungary, Sept. 9, 1942; Head of the Serology laboratory. At Johan Bela Nat. Inst. Hygiene, 1980; s. Pal Bozsik and Maria Klinga; m. Marta Schleer, Dec. 4, 1968; children: Bela, Andras Pal, Attila Peter. MD, Semmelweis Med. U., Budapest, 1966. Worker Lenin Metall. Works, Miskolc, Hungary, 1960-61; pathologist St. Istvan Mcpl. Hosp., Budapest, 1966-74; rschr. Nat. Inst. Hygiene, Budapest, 1974-99; head Lyme ctr. St Rokus Hosp., Budapest, 1999; ret., 1999; researcher at Lyme Clinic, Budapest 2002-2003.03. Head of Serology Laboratory at Johan Bela Nat. Inst. Hygiene, 1980-99. Author: (in Hungarian) Rules of Ticks, 1995, Advice on Tick Diseases, 1997. Founder, Med, Sec. Head of, Lyme Borreliosis Foundation., Budapest. Mem. N.Y. Acad. Sci. and Soc. for Christian Physicians, Roman Catholic.

Achievements include proposing the worldwide accepted technical term for Lyme Disease and Related Disorders that is Lyme Borreliosis in 1985 at the 2nd World Conference, proposing a diagnostic and therapeutic scheme for Lyme borreliosis seronegatives, and new hypothesis for pathogenesis of Lyme borreliosis respectively. The diagnostic method was Patented in 2004.

New method for evaluating the immunohemolytic activity of Complement with modified method was developed and new hypothesis for the structure of Membrane Attack Complex (C5b-C9) was constructed during 1974-1984. Based on the studies of the Complement system a new micro-method for complement fixation method was elaborated.
Office: Lyme Borreliosis Foundation Hungary
Email: bpbozsik at freestart.hu

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